목적: Regeneration of the facial nerve using low level laser therapy (LLLT) has been rarely reported. Polydeoxyribonucleotides (PDRN), a mixture of short dexoribonucleotide polymers (non-toxic agonist and no antigenic properties is a well-known stimulator of cell growth that accelerates cell proliferation and can help with the wound healing. In this study, we examined synergistic effect of topical sustained release of PDRN/F-127 and LLLT on facial nerve regeneration in the crush injury induced facial nerve paralysis of rat. 방법:The main trunk was crushed for one minute with a hemostat. Animal were divided 5 groups; control group (n=4), group I (pluronic F-127 only, n=4), group II (pluronic F-127/PDRN, n=4), group III (pluronic F-127 + LLLT, n=4), and group IV (pluronic F-127/PDRN + LLLT, n=4). The recovery of vibrissa fibrillation, action potential and facial nerve blood flow (FNBF) were measured. 결과:Group IV demonstrated significantly better recovery compared to the other groups. After the intervention, significant differences in vibrissa fibrillation values were observed at all time points (p = 0.0028) according to the repeated one-way ANOVA. Group IV showed the most notable recovery in facial muscle action potential and FNBF compared to the other groups. 결론:These findings suggest that PDRN and LLLT may work together synergistically to enhance peripheral nerve regeneration. Future research should investigate the underlying molecular mechanisms and evaluate the potential clinical applications of this combined treatment strategy.