목적: In this study, we report on the development of a peripheral nerve organoid composed of human induced pluripotent stem cell (iPSC)-derived motor neurons and primary human Schwann cells, utilizing a microfluidic device. 방법:The process begins with forming human iPSCs into spheroids, followed by their differentiation into motor neurons using a 3D protocol with small molecules. These differentiated motor neuron spheroids are then transferred to a microfluidic device for co-culture with primary human Schwann cells, with a particular focus on myelinated axon formation. 결과:An optimized combination of inducing and patterning factors (SB431542, LDN-193189, retinoic acid, and smoothened agonist) along with acceleration factors (SU5402 and DAPT) facilitates rapid differentiation of human iPSCs. The use of CHIR 99021 led to an increased expression of Nesting, indicating an enhancement in neural induction compared to controls without CHIR. This was observed alongside the robust development of the organoid body and the augmented formation of neuronal dendrites. Subsequently, myelinated axons naturally emerge from the spheroid, forming a fascicle withing the microchannel of the chip. 결론:This study highlights the significant role of CHIR 99021 in enhancing the development of our nerve organoid model. Our peripheral nerve organoid is expected to be a valuable tool for offering a promising approach for research in facial nerve regeneration.