목적: Olfactory dysfunction, such as anosmia or hyposmia, is a recognized symptom of ciliopathies, which are multiple genetic disorders arising from abnormalities in cilia assembly, maintenance, and/or function in both humans and animal models. The positioning of the centrioles (basal bodies) to the OSN knob during OSN development is crucial for ciliogenesis, where cilia form on the knob surface and extend in the direction of the airway. However, the mechanism by which the centriole- derived basal body positions itself on the cell surface to serve as the template for cilium formation remains unclear. 방법:1. Since homozygous knockout of Kif3a in mice is embryonic lethal, we generated mice with OMP-specific inactivation of Kif3a induced by Cre- recombinase under the olfactory marker protein (OMP) promoter (OMP- cre;Kif3aflox/flox). 2. We performed primary phenotyping to investigate whether OMP- cre;Kif3aflox/flox, generated through odorant behavior tests, perceive odors. Additionally, we conducted secondary phenotyping using electro- olfactogram to examine the functional abnormalities of OMP- cre;Kif3aflox/flox OSNs. 3. We investigated histopathological abnormalities such as knob formation, ciliogenesis, and centriole positioning in OMP- cre;Kif3aflox/flox mouse OSNs using transmission electron microscopy (TEM), scanning electron microscopy (SEM), and immunohistochemistry. 4. We induced ciliogenesis in cultured cells and subsequently treated them with a microtubule depolymerizing reagent to investigate whether centriole migration is closely related to microtubules associated with Kif3a in our in vitro study. 결과:We generated mice with OMP-specific inactivation of Kif3a induced by Cre-recombinase under the olfactory marker protein (OMP) promoter. This model exhibited impaired smell perception, as evidenced by odorant behavior tests and electro-olfactogram recordings. Histopathological analysis revealed severely abnormal knob formation and significant defects in cilia formation in the OSNs. Notably, numerous centrioles were found to be ectopically localized on the OSN dendrite. Furthermore, an in vitro study using nocodazole treatment demonstrated that centriole positioning during ciliogenesis depends closely on microtubules associated with Kif3a function. 결론:These findings suggest that the lack of Kif3a leads to noticeable disruptions in centriole positioning, ultimately impacting the intricate process of olfactory sensory neuron development.