목적: Stem cells play a crucial role in tissue regeneration and repair, and their ability to maintain stemness is essential for effective therapeutic applications. These cells are sensitive to gravitational variations, which can significantly impact their stemness attributes. The study aimed to investigate the influence of simulated microgravity on the stemness and regenerative potential of human minor salivary gland stem cells (hMSGSCs), with a focus on their viability for salivary gland tissue regeneration therapies. 방법:hMSGSCs were subjected to two different culture conditions: three days in microgravity followed by three days in normal gravity (1G), and a control group continuously maintained in 1G. Post-culture, a comprehensive analysis was conducted, including assessments of cell proliferation, cell cycle progression, reactive oxygen species (ROS) levels, apoptosis, and expression of stemness markers. 결과:Exposure to three days of microgravity led to a decrease in cell proliferation, an increase in ROS levels, and elevated early apoptosis in hMSGSCs compared to cells exposed to 1G. Cell cycle analysis revealed a reduction in the G0/G1 phase and S phase arrest in the microgravity- exposed cells. Intriguingly, when both groups were subsequently exposed to 1G for three days, the microgravity-exposed cells exhibited increased proliferation and decreased ROS levels. Moreover, these cells showed enhanced expression of stemness markers, though no significant difference in the cell cycle was observed between the two groups. 결론:Notably, local injection of microgravity-exposed hMSGSCs into irradiated mouse salivary glands resulted in the functional recovery of the glands, indicating the potential therapeutic efficacy of these cells. In conclusion, the findings suggest that microgravity exposure may prime hMSGSCs for enhanced regenerative capabilities, providing promising insights for future salivary gland tissue regeneration therapies. However, further research is warranted to elucidate the underlying mechanisms and optimize the applications of this novel approach.