목적: The purpose of this study was to develop a CMCSCC-1 cell line for head and neck cancer research into new therapies for head and neck squamous cell carcinoma (SCC). 방법:The CMCSCC-1 cell line was isolated from a primary oral tongue tumor specimen of a female patient. Tumor cells were evaluated for biomarkers expression by Western blots, RT-PCR, FACs, and immunostaining. Cell proliferation in response to radiation was measured by the WST-8 assay. 결과:The characterization analyses revealed a typical epithelial morphology a doubling time of approximately 24h, high tumorigenicity in immunodeficient mice, and upregulated biomarkers. CMCSCC-1 cells were negative for human papillomavirus (HPV) infection, but more sensitive to radiation compared with those FaDu cell line. Conclusion.CMCSCC-1, a novel oral tongue SCC cell line was established. It will help to in the elucidation of the molecular pathogenesis of HPV-negative, radiosensitive tumors. 결론:CMCSCC-1, a novel oral tongue SCC cell line was established. It will help to in the elucidation of the molecular pathogenesis of HPV-negative, radiosensitive tumors.