목적: Various types of salivary gland (SG)-resident stem/progenitor cells have been isolated and suggested for candidates of stem cell therapy for restoration of SG hypofunction. Homogenous clonal cells from a small amount of SG biopsy should be obtained and expanded rapidly ex vivo for clinical trials in human. Herein, we describe a method of rapid enrichment of human SG-derived clonal stem cells and characterize their stem cell properties. 방법:We isolated human SG-derived clonal stem cells (hSGSCs) by a modified subfractionation culture method. Three single cell clones were chosen, expanded, and examined for their stem cell properties. 결과:hSGSCs showed MSCs-like characteristics in terms of their marker expression and differentiation potential into fat, bone, and cartilage cell lineages with some different potential in each clones. They also exhibited epithelial-related characteristics and showed the ability to differentiate into α-amylase-producing and hepatocyte-like cells. In addition, they showed immunosuppressive activity in vitro with some different potency again. 결론:These results showed that by this protocol, we can obtain enriched single cell clone-derived SGSCs from a small biopsied SG pieces, and they have multipotent stem cell properties capable of differentiating into mesenchymal and epithelial lineage cells. Each clone possesses some different MSC characteristics and immunomodulatory activities.