목적: The amniotic membrane is a favorable biomaterial to apply the field of tissue engineering because of its biological properties. Human amniotic membrane consists of two-layered sheet, which contains numerous growth factors, cytokines and other active substrances. In this study, we explored possibility of amnion membrane extracts (AME) and amnion/chorion membrane extracts (A/CME) to improve osteogenic differentiation of osteoblast like cells (MG-63 cells). 방법:MG-63 cells were cultured in OIM (Osteogenic Induction Medium) combination with or without 100g/ml of AME and A/CME, respectively. To determine the early and late differentiation of osteogenesis, Alkaline phosphatase (ALP) activity and calcium deposition were measured at different time intervals of 3, 7, 10 day and 24 day respectively. The mRNA expression of osteocalcin (OCN), osteopontin (OPN), runt domain-containing transcription factor (RUNX2) and osterix (OSX) genes were also quantified as specific marker for osteogenic differentiation. 결과:This in vitro study found that A/CME improves ALP activity, mRNA expression of osteogenic marker genes as well as mineralization under osteogenic inducing condition. Especially, A/CME contains specific growth factors related to osteogenesis such as fibroblast growth factors (FGFs) and transforming growth factors (TGF-) which might stimulate the osteogenic differentiation of MG-63 cells more than AME. 결론:These results indicate that A/CME is capable of providing growth factors and other substrates for osteogenic differentiation, which significantly increasing the efficacy of osteogenesis on MG-63 cells. Taken together, this study suggests that human A/CM extract have therapeutic potential to apply in bone regeneration as a promising biomaterial.