목적: Keloid is the overgrowth fibrous formation of scar and therapeutic treatments ofkeloid including occlusive dressings, compression therapy, intralesional corticosteroid injections, crysurgery, excision, radiation therapy, laser therapy have tried until now, but no single treatment of keloid is best of choice. Non-thermal atmospheric pressure plasma (NTP) is a novel treatment method for various applications such as wound healing, blood coagulation and cancer therapy. The aim of our study was to identify the effect of this novel method on keloid which is refractory disease. 방법:We used western blots to compared normal fibroblast (NF) and keloid fibroblast (KF) about expression the level of epidermal growth factor receptor (EGFR), signal transducer and activator of transcription 3 (STAT- 3), collagen type1 and to identify the role of EGFR and STAT-3 after NTP treatment. To evaluate NTP toxicity on keloid, we used Annexin V/propidium iodide staining. We also used immunocystochemistry to figure out NTP suppression on collagen formation. Wound healing assay was used for visualize fibroblast migration which is key role in wound healing. 결과:EGFR and STAT-3 feature significant disparity expression between normal skin and keloid. NTP does not induce cell death both in NF and KF. NTP acts oppositely on NF and KF, NTP suppressed collagen 1, collagen 3 and TGF-b expression only in KF. Wound healing assay results show NTP increasing migration of NF but decreasing migration of KF. We figured out that NTP down-regulate STAT-3, and reduce KF migration 결론:These findings show the therapeutic potential of NTP in keloid. To our knowledge, this is the first report of a possible mechanism of the therapeutic effect of NTP on keloid which reduced migration via STAT-3 downregulation.